After performing FBA, thermodynamic analysis on the heterologous pathways generated by Retropath2.0 this tool normalizes the different values from each heterologous pathway, and performs a weighted sum function using the following attributes:
- Length of the heterologous pathways
- Flux to the target compound
- Thermodynamic feasibility of the heterologous pathway
- Sum of the reaction rule scores
Given a collection or a single SBML file, this tool generates a CSV file output with all pathway information from the analysis tools (thermodynamics, FBA, etc). You can also specify the heterologous reactions and chemical species by providing the name of the heterologous pathway or SBML file ID.