Muconic Acid Population Sample

Studies: Assemble parts, Transformation of production host

Assays: Automated HT spreadind using colony picker with compartementalized Qtrays, Automated recovery of transformant and distribution in appropriate format, Cell preparation from stock solution, Colony picking, Colony picking for verification of the genetic material, In vitro assembly of the DNA construct, Incubation of cells in recovery medium, Make glycerol stock for part storage, Make glycerol stock for strain storage, Order synthetic DNA, Preparation and distribution of recovery medium in microplate format, Preparation and distribution of transformation medium in microplate format, Prepare cultivation media for cultivation after colony picking, Prepare cultivation media for making glycerol stocks, Prepare cultivation media for transformation step, Prepare liquid cultivation medium for transformation, Prepare solid cultivation medium for transformation, Prepare template DNA for verification of the genetic material, Prepare transformation competent cells of the cloning host organism, Recombinant clone screening and selection (depends on transformation imp..., Select transformation conditions, Transform genetic material into cloning host organism, Transformation, Transformation, Verify by recombinant clone screening, Withdraw parts from storage

Studies: Metabolic strain design

Assays: Change medium, Change temperature, Estimate market cost of substrate, Estimate toxicity for host, Measure uptake kinetics, Obtain annotated genome for production host

Investigation: Strain engineering

Assays: No Assays

Investigation: Strain engineering

Assays: Automated HT spreadind using colony picker with compartementalized Qtrays, Automated recovery of transformant and distribution in appropriate format, Cell preparation from stock solution, Colony picking, Colony picking for verification of the genetic material, In vitro assembly of the DNA construct, Incubation of cells in recovery medium, Make glycerol stock for part storage, Make glycerol stock for strain storage, Order synthetic DNA, Preparation and distribution of recovery medium in microplate format, Preparation and distribution of transformation medium in microplate format, Prepare cultivation media for cultivation after colony picking, Prepare cultivation media for making glycerol stocks, Prepare cultivation media for transformation step, Prepare liquid cultivation medium for transformation, Prepare solid cultivation medium for transformation, Prepare template DNA for verification of the genetic material, Prepare transformation competent cells of the cloning host organism, Recombinant clone screening and selection (depends on transformation imp..., Select transformation conditions, Transform genetic material into cloning host organism, Transformation, Transformation, Verify by recombinant clone screening, Withdraw parts from storage

Investigation: Computational strain design

Assays: Change medium, Change temperature, Estimate market cost of substrate, Estimate toxicity for host, Measure uptake kinetics, Obtain annotated genome for production host

Investigation: Strain engineering

Study: Transformation of production host

Organisms: No organisms

Protocols: No Protocols

Data files: No Data files

Investigation: Strain engineering

Study: Transformation of production host

Organisms: No organisms

Protocols: No Protocols

Data files: No Data files

Investigation: Strain engineering

Study: Transformation of production host

Organisms: No organisms

Protocols: No Protocols

Data files: No Data files

Investigation: Strain engineering

Study: Transformation of production host

Organisms: No organisms

Protocols: No Protocols

Data files: No Data files

Investigation: Strain engineering

Study: Transformation of production host

Organisms: No organisms

Protocols: No Protocols

Data files: No Data files

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Investigations: No Investigations

Studies: No Studies

Assays: No Assays