Muconic Acid Population Sample
OverviewProgramme: IBISBA 2020 f2f
SEEK ID: https://hub.ibisba.eu/projects/34
Public web page: Not specified
Organisms: Pseudomonas putida
IBISBA Project managers: No Project managers for this Project
Project created: 6th Jul 2020
Related items
https://orcid.org/0000-0003-3549-8649
Files and projects to support the IBISBA 2020 f2f
Projects: An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data, PopulationTest2, Muconic Acid Development from Yeast, Muconic Acid Population Sample, Demo Project by Munazah, demo project 2
Web page: Not specified
Submitter: Stefan Helfrich
Studies: Assemble parts, Transformation of production host
Assays: Automated HT spreadind using colony picker with compartementalized Qtrays, Automated recovery of transformant and distribution in appropriate format, Cell preparation from stock solution, Colony picking, Colony picking for verification of the genetic material, In vitro assembly of the DNA construct, Incubation of cells in recovery medium, Make glycerol stock for part storage, Make glycerol stock for strain storage, Order synthetic DNA, Preparation and distribution of recovery medium in microplate format, Preparation and distribution of transformation medium in microplate format, Prepare cultivation media for cultivation after colony picking, Prepare cultivation media for making glycerol stocks, Prepare cultivation media for transformation step, Prepare liquid cultivation medium for transformation, Prepare solid cultivation medium for transformation, Prepare template DNA for verification of the genetic material, Prepare transformation competent cells of the cloning host organism, Recombinant clone screening and selection (depends on transformation imp..., Select transformation conditions, Transform genetic material into cloning host organism, Transformation, Transformation, Verify by recombinant clone screening, Withdraw parts from storage
Submitter: Stefan Helfrich
Investigation: Strain engineering
Assays: Automated HT spreadind using colony picker with compartementalized Qtrays, Automated recovery of transformant and distribution in appropriate format, Cell preparation from stock solution, Colony picking, Colony picking for verification of the genetic material, In vitro assembly of the DNA construct, Incubation of cells in recovery medium, Make glycerol stock for part storage, Make glycerol stock for strain storage, Order synthetic DNA, Preparation and distribution of recovery medium in microplate format, Preparation and distribution of transformation medium in microplate format, Prepare cultivation media for cultivation after colony picking, Prepare cultivation media for making glycerol stocks, Prepare cultivation media for transformation step, Prepare liquid cultivation medium for transformation, Prepare solid cultivation medium for transformation, Prepare template DNA for verification of the genetic material, Prepare transformation competent cells of the cloning host organism, Recombinant clone screening and selection (depends on transformation imp..., Select transformation conditions, Transform genetic material into cloning host organism, Transformation, Transformation, Verify by recombinant clone screening, Withdraw parts from storage
Submitter: Stefan Helfrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Strain engineering
Submitter: Stefan Helfrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Strain engineering
Submitter: Stefan Helfrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Strain engineering
Submitter: Stefan Helfrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Strain engineering
Submitter: Stefan Helfrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Strain engineering
