SOPs

What is a SOP?
32 SOPs visible to you, out of a total of 130
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

This protocol describes the procedure of conjugation of pK18mobSacB suicide plasmid containing fragments UP and DOWN that delimit the recombination region from E. coli S17λpir to P. putida cells.

Creators: Felipe de Salas, Laura de Eugenio, Beatriz Galán

Submitter: Felipe de Salas

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Leaf agroinfiltration is a method used in plant biology and biotechnology to induce transient expression of genes in leaf tissue, in order to produce a desired protein. In the method, a suspension of Agrobacterium tumefaciens, which contains a plasmid with the gene of interest, is introduced into a plant leaf by direct injection, whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main benefit of agroinfiltration, when compared to the more traditional ...

Creators: Emanuela Pedrazzini, Emanuela Pedrazzini

Submitter: Emanuela Pedrazzini

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

GEM preparation

Scale-up

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Template to collect input data for execution of LEARN step

This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.

All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...

Creator: Rita Volkers

Submitter: Rita Volkers

Powered by
(v.1.14.2)
Copyright © 2008 - 2023 The University of Manchester and HITS gGmbH
IBISBA is a pan-European research infrastructure that is currently funded by the EU Horizon 2020 projects
IBISBA 1.0 (grant agreement number 730976) and PREP-IBISBA (grant agreement number 871118).
Registering data or other knowledge assets on this platform is the sole responsibility of Users.
IBISBA cannot be held responsible for misuse or misappropriation of data and assets belonging to a Third Party..