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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR02 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR02 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/909 | ||||||||
| OR | |||||||||
| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| I1 | 20 | 2018-05-16T7:58:0+0000 | 0.15653846153847933 | 0.04 | 9.629999999999999 | 10.478 | 0.124 | 5.096 | 298 | |||
| I2 | 25 | 2018-05-16T9:3:0+0000 | 0.0035714285713726673 | 0.07399999999998993 | 0.045 | 9.61 | 298 | |||||
| I3 | 20 | 2018-05-16T10:4:0+0000 | 0.11281250000000353 | 0.05 | 9.57 | 8.533 | 0.128 | 4.21 | 298 | |||
| I4 | 25 | 2018-05-16T11:55:0+0000 | 0.0892857142856648 | 0.06129629629632778 | 0.08 | 9.31 | 9.02 | 0.136 | 4.461 | 298 | ||
| I5 | 25 | 2018-05-16T13:0:0+0000 | 0.023214285714279193 | 0.05323529411766928 | 0.12 | 9.389999999999999 | 7.718 | 0.144 | 3.858 | 298 | ||
| I6 | 20 | 2018-05-16T13:53:0+0000 | 0.3176388888889159 | 0.14 | 9.350000000000001 | 8.66 | 0.158 | 4.334 | 298 | |||
| I7 | 22.5 | 2018-05-16T15:0:0+0000 | 0.23095238095246653 | 0.22211111111113033 | 0.315 | 9.3 | 6.97 | 0.171 | 3.514 | 298 | ||
| I8 | 22.5 | 2018-05-16T16:13:0+0000 | 0.4607142857142905 | 0.23752688172042474 | 0.79 | 8.53 | 7.715 | 0.242 | 4.147 | 298 | ||
| I9 | 22 | 2018-05-16T17:3:0+0000 | 0.5642857142856839 | 0.758194444444397 | 7.95 | 6.587 | 0.281 | 3.886 | 298 | |||
| I10 | 16 | 2018-05-16T17:50:0+0000 | 1.2821428571428508 | 1.1959999999999305 | 2.8000000000000003 | 6.760000000000001 | 5.77 | 0.337 | 4.01 | 298 | ||
| I11 | 5 | 2018-05-16T19:36:0+0000 | 12.25 | 0.365 | 0.391 | 3.699 | 298 | |||||
| I12 | 10 | 2018-05-16T20:20:0+0000 | 6.1750000000000105 | 6.382051282051302 | 17.25 | 0.011 | 0.144 | 3.911 | 298 | |||
| I13 | 5 | 2018-05-16T21:57:0+0000 | 12.871428571428519 | 11.194999999999844 | 29.4 | 0 | 0.166 | 298 | ||||
| I14 | 5 | 2018-05-16T22:45:0+0000 | 16.071428571428545 | 15.497500000000164 | 36.5 | 0 | 2.841 | 298 | ||||
| I15 | 7 | 2018-05-16T23:37:0+0000 | 19.550000000000086 | 19.730000000000025 | 45.8 | 0.01 | 3.068 | 298 | ||||
| I16 | 59 | 2018-05-17T0:13:0+0000 | 23.07142857142841 | 24.42000000000011 | 55.400000000000006 | 0 | 2.702 | 0 | 0.228688920329017 | 298 | ||
| I17 | 60 | 2018-05-17T1:30:0+0000 | 23.982142857142797 | 26.27500000000005 | 59.2 | 0 | 2.885 | 0.32441277565430265 | 0.5364377598138621 | 298 | ||
| I18 | 60 | 2018-05-17T3:15:0+0000 | 24.714285714285687 | 25.800000000000267 | 53.00000000000001 | 0.011 | 2.62 | 0.23194522985139734 | 0.1682769997662573 | 298 | ||
| I19 | 60 | 2018-05-17T5:22:0+0000 | 25.246428571428385 | 27.125000000000288 | 62.1 | 0.011 | 2.406 | 0.43939658381477764 | 0.8278071751751994 | 298 | ||
| I20 | 61 | 2018-05-17T7:22:0+0000 | 27.319999999999567 | 67.2 | 0.005 | 2.256 | 0.5877168901301933 | 1.3672982147077921 | 298 | |||
| I21 | 60 | 2018-05-17T9:19:0+0000 | 27.314285714285674 | 28.115000000000112 | 70 | 0 | 3.038 | 1.272824681403358 | 1.0997631705662052 | 298 | ||
| I22 | 58 | 2018-05-17T10:22:0+0000 | 27.653571428571393 | 26.956666666666518 | 71.8 | 0.006 | 3.038 | 1.2354828854020108 | 0.5186263845866387 | 298 | ||
| I23 | 58 | 2018-05-17T11:23:0+0000 | 28.039285714285747 | 28.203333333333358 | 74.2 | 0.009 | 2.559 | 1.6017797213187899 | 1.0542166429530662 | 298 | ||
| I24 | 230 | 2018-05-17T12:21:0+0000 | 28.417857142857116 | 27.984999999999705 | 73.2 | 0 | 2.51 | 2.577513918464948 | 3.034546426088498 | 298 | ||
