Protocols
This protocol describes the procedure of conjugation of pK18mobSacB suicide plasmid containing fragments UP and DOWN that delimit the recombination region from E. coli S17λpir to P. putida cells.
Creators: Felipe de Salas, Laura de Eugenio, Beatriz Galán
Submitter: Felipe de Salas
Leaf agroinfiltration is a method used in plant biology and biotechnology to induce transient expression of genes in leaf tissue, in order to produce a desired protein. In the method, a suspension of Agrobacterium tumefaciens, which contains a plasmid with the gene of interest, is introduced into a plant leaf by direct injection, whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main benefit of agroinfiltration, when compared to the more traditional ...
Creators: Emanuela Pedrazzini, Emanuela Pedrazzini
Submitter: Emanuela Pedrazzini
