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29 Protocols visible to you, out of a total of 113

This protocol describes the procedure of conjugation of pK18mobSacB suicide plasmid containing fragments UP and DOWN that delimit the recombination region from E. coli S17λpir to P. putida cells.

Creators: Felipe de Salas, Laura de Eugenio, Beatriz Galán

Submitter: Felipe de Salas

Leaf agroinfiltration is a method used in plant biology and biotechnology to induce transient expression of genes in leaf tissue, in order to produce a desired protein. In the method, a suspension of Agrobacterium tumefaciens, which contains a plasmid with the gene of interest, is introduced into a plant leaf by direct injection, whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main benefit of agroinfiltration, when compared to the more traditional ...

Creators: Emanuela Pedrazzini, Emanuela Pedrazzini

Submitter: Emanuela Pedrazzini

This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.

All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...

Creator: Rita Volkers

Submitter: Rita Volkers

Template to collect input data for execution of LEARN step


Pathways elimination

GEM preparation

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