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8 Investigations visible to you, out of a total of 19

Computational strain design

Muconic Acid Population Sample

No description specified

Snapshots: No snapshots

Studies: Metabolic strain design

Assays: Change medium, Change temperature, Estimate market cost of substrate, Estimate toxicity for host, Measure uptake kinetics, Obtain annotated genome for production host

Created: 6th Jul 2020 at 15:04

In-silico prediction and mass spectrometric validation of the Aspergillus niger secretome

An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data

No description specified

Snapshots: No snapshots

Studies: Culture experiments, In-Silico Prediction, Metabolic model analysis, Validation of the secretome of Aspergillus niger

Assays: GPI Anchor Predictions, Growth experiment, Identify enzymes from spectra, Liquid chromatography tandem mass spectrometry, Mass Spectrometry Analysis, Obtain metabolic model, Obtain proteomes, Orthology identification, Signal Peptide Predictions

Created: 18th May 2020 at 14:58, Last updated: 19th May 2020 at 12:36

Strain engineering

TestProjectForPopulation

No description specified

Snapshots: No snapshots

Studies: Assemble parts, Transformation of production host

Assays: Automated HT spreadind using colony picker with compartementalized Qtrays, Automated recovery of transformant and distribution in appropriate format, Cell preparation from stock solution, Colony picking, Colony picking for verification of the genetic material, In vitro assembly of the DNA construct, Incubation of cells in recovery medium, Make glycerol stock for part storage, Make glycerol stock for strain storage, Order synthetic DNA, Preparation and distribution of recovery medium in microplate format, Preparation and distribution of transformation medium in microplate format, Prepare cultivation media for cultivation after colony picking, Prepare cultivation media for making glycerol stocks, Prepare cultivation media for transformation step, Prepare liquid cultivation medium for transformation, Prepare solid cultivation medium for transformation, Prepare template DNA for verification of the genetic material, Prepare transformation competent cells of the cloning host organism, Recombinant clone screening and selection (depends on transformation imp..., Select transformation conditions, Transform genetic material into cloning host organism, Transformation, Transformation, Verify by recombinant clone screening, Withdraw parts from storage

Created: 2nd Jul 2020 at 15:15

Strain engineering

PopulationTest2

No description specified

Snapshots: No snapshots

Studies: Assemble parts, Transformation of production host

Created: 2nd Jul 2020 at 16:50

Strain engineering

Muconic Acid Population Sample

No description specified

Snapshots: No snapshots

Studies: Assemble parts, Transformation of production host

Created: 20th Aug 2020 at 11:17

Strain Engineering for Yeast

Muconic Acid Developemnt from Yeast

No description specified

Snapshots: No snapshots

Studies: Assemble Parts, Transformation of production host

Assays: Colony Picking for verification of Genetic Material, Prepare Genetic material for Transformation, Prepare transformation of competent cells, Procure Synthetic DNA, Recombinant Clone Screening and Selection, Transformation and Colony Picking, Verify by recombinant clone screening

Created: 5th Jul 2020 at 14:55

Strain Management for Yeast

Muconic Acid Developemnt from Yeast

No description specified

Snapshots: No snapshots

Studies: Strain Transfer for Yeast

Assays: Deposit Strain to Storage, Strain Revitalization

Created: 5th Jul 2020 at 14:40, Last updated: 5th Jul 2020 at 14:42