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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR03 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/910 | ||||||||
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| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| J1 | 20 | 2018-05-16T8:6:0+0000 | 0.169499999999978 | 0.025 | 9.629999999999999 | 8.228 | 0.119 | 4.191 | 298 | |||
| J2 | 21 | 2018-05-16T9:3:0+0000 | 0.11299999999998533 | 0.05 | 9.61 | 8.092 | 0.123 | 4.076 | 298 | |||
| J3 | 23 | 2018-05-16T10:4:0+0000 | 0.08906250000001448 | 0.05 | 9.57 | 8.374 | 0.129 | 4.286 | 298 | |||
| J4 | 20 | 2018-05-16T11:52:0+0000 | 0.042857142857185726 | 0.20500000000001073 | 0.085 | 9.389999999999999 | 7.7 | 0.134 | 3.96 | 298 | ||
| J5 | 21 | 2018-05-16T13:1:0+0000 | 0.014285714285728572 | 0.054285714285720994 | 0.10999999999999999 | 9.350000000000001 | 8.152 | 0.142 | 4.155 | 298 | ||
| J6 | 20 | 2018-05-16T13:58:0+0000 | 0.0553571428570297 | 0.1263541666666479 | 0.155 | 9.31 | 7.902 | 0.158 | 4.067 | 298 | ||
| J7 | 22 | 2018-05-16T15:0:0+0000 | 0.25595238095231315 | 0.1675555555555282 | 0.325 | 9.3 | 8.094 | 0.185 | 4.237 | 298 | ||
| J8 | 24 | 2018-05-16T16:13:0+0000 | 0.42500000000008803 | 0.42117647058825164 | 0.905 | 8.53 | 7.947 | 0.232 | 4.32 | 298 | ||
| J9 | 15 | 2018-05-16T17:3:0+0000 | 0.5214285714284982 | 0.6382352941176747 | 1.47 | 8.36 | 7.273 | 0.276 | 4.195 | 298 | ||
| J10 | 16 | 2018-05-16T17:50:0+0000 | 1.0035714285714212 | 1.0854761904761645 | 2.96 | 7.54 | 5.661 | 0.33 | 3.792 | 298 | ||
| J11 | 298 | |||||||||||
| J12 | 8 | 2018-05-16T20:18:0+0000 | 5.957142857142867 | 6.494 | 16.400000000000002 | 0.215 | 0.295 | 0.366 | 3.944 | 298 | ||
| J13 | 13 | 2018-05-16T22:0:0+0000 | 12.128571428571426 | 10.977000000000015 | 29.15 | 0 | 0 | 0.153 | 3.275 | 298 | ||
| J14 | 7 | 2018-05-16T22:44:0+0000 | 16.207142857142852 | 16.246153846153696 | 39.7 | 0.001 | 0 | 0.163 | 3.163 | 298 | ||
| J15 | 12 | 2018-05-16T23:37:0+0000 | 21.774999999999988 | 21.016666666666833 | 51 | 0.015 | 0 | 3.375 | 298 | |||
| J16 | 59 | 2018-05-17T0:13:0+0000 | 26.35714285714281 | 24.869999999999948 | 63.5 | 0.012 | 0 | 2.659 | 0 | 0.07416873810681106 | 298 | |
| J17 | 57 | 2018-05-17T1:30:0+0000 | 26.92857142857148 | 27.855000000000185 | 67.4 | 0.013 | 0 | 3.212 | 0.3177678298217808 | 1.4092060240294102 | 298 | |
| J18 | 60 | 2018-05-17T3:15:0+0000 | 25.41785714285721 | 28.084999999999916 | 67.4 | 0 | 0 | 3.353 | 0.4126104156784756 | 0.9394706826862734 | 298 | |
| J19 | 60 | 2018-05-17T5:22:0+0000 | 27.317857142857207 | 23.925000000000196 | 67 | 0.013 | 0 | 2.929 | 0.46584767025032514 | 0.34612077783178496 | 298 | |
| J20 | 60 | 2018-05-17T7:22:0+0000 | 27.735714285714312 | 34.18499999999991 | 74.39999999999999 | 0.01 | 0 | 2.772 | 0.7367263838869168 | 0.420289515938596 | 298 | |
| J21 | 69 | 2018-05-17T9:18:0+0000 | 31.153571428571244 | 31.43000000000029 | 81.39999999999999 | 0 | 0 | 3.028 | 1.2861185305328178 | 0.7664102937703811 | 298 | |
| J22 | 225 | 2018-05-17T10:22:0+0000 | 30.203571428571443 | 30.821666666666747 | 81.6 | 0.005 | 0 | 3.009 | 2.6290553628074136 | 2.0025559288838988 | 298 | |
