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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR05 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR05 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 37°C for 6 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/912 | ||||||||
| OR | |||||||||
| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| L1 | 27 | 2018-05-29T7:20:0+0000 | 0.03928571428573375 | 0.04814814814814284 | 0.24 | 10.3 | 10.437 | 0.131 | 5.8 | 326 | ||
| L2 | 19 | 2018-05-29T8:20:0+0000 | 0.13928571428575445 | 0.04347826086957973 | 0.42000000000000004 | 10.2 | 10.233 | 0.157 | 5.703 | 326 | ||
| L3 | 19 | 2018-05-29T9:1:0+0000 | 0.1328787878787904 | 0.685 | 9.459999999999999 | 9.897 | 0.201 | 5.655 | 326 | |||
| L4 | 21 | 2018-05-29T10:3:0+0000 | 0.44285714285711 | 0.4653846153846239 | 1.44 | 8.98 | 9.372 | 0.299 | 5.69 | 326 | ||
| L5 | 21 | 2018-05-29T10:29:0+0000 | 0.6816071428571276 | 2.05 | 8.17 | 7.779 | 0.314 | 5.016 | 326 | |||
| L6 | 19 | 2018-05-29T10:57:0+0000 | 1.0678571428571602 | 0.9277500000000494 | 2.8100000000000005 | 7.71 | 8.13 | 0.39 | 5.675 | 326 | ||
| L7 | 14 | 2018-05-29T11:30:0+0000 | 1.0285714285714265 | 1.4302777777778095 | 4.34 | 7.24 | 6.981 | 0.45 | 5.743 | 326 | ||
| L8 | 12 | 2018-05-29T12:0:0+0000 | 2.539285714285697 | 2.2045000000000314 | 6.36 | 5.08 | 5.438 | 0.55 | 5.763 | 326 | ||
| L9 | 15 | 2018-05-29T12:30:0+0000 | 3.7285714285714304 | 3.41107142857146 | 9.26 | 3.08 | 3.34 | 0.585 | 5.796 | 326 | ||
| L10 | 10 | 2018-05-29T12:59:0+0000 | 4.485714285714332 | 4.0855555555554295 | 11.92 | 0.6 | 1.209 | 0.71 | 5.759 | 326 | ||
| L11 | 12 | 2018-05-29T13:50:0+0000 | 8.025000000000075 | 7.474500000000184 | 21.05 | 0.012 | 0.238 | 5.644 | 326 | |||
| L12 | 12 | 2018-05-29T14:50:0+0000 | 11.517857142857183 | 10.262499999999974 | 26.200000000000003 | 0.159 | 1.179 | 5.375 | 326 | |||
| L13 | 11 | 2018-05-29T15:20:0+0000 | 14.978571428571541 | 12.863124999999975 | 36.5 | 0.012 | 5.232 | 326 | ||||
| L14 | 8 | 2018-05-29T16:3:0+0000 | 19.46071428571426 | 16.852499999999935 | 47.199999999999996 | 0.011 | 5.167 | 326 | ||||
| L15 | 8 | 2018-05-29T16:41:0+0000 | 22.09642857142853 | 20.738750000000028 | 59.39999999999999 | 0.008 | 4.489 | 326 | ||||
| L16 | 63 | 2018-05-29T17:24:0+0000 | 28.428571428571473 | 20.944999999999993 | 74.39999999999999 | 0.015 | 4.116 | 0 | 0.6124383844200939 | 326 | ||
| L17 | 50 | 2018-05-29T18:16:0+0000 | 29.314285714285692 | 27.660000000000018 | 71.19999999999999 | 0.011 | 4.306 | 0.06621581651871132 | 1.5923397994922441 | 326 | ||
| L18 | 52 | 2018-05-29T19:22:0+0000 | 30.06428571428577 | 28.927499999999995 | 79.4 | 0 | 3.955 | 0.1537895837898565 | 0.8727246977986338 | 326 | ||
| L19 | 52 | 2018-05-29T20:20:0+0000 | 30.938095238095226 | 29.09499999999987 | 84.2 | 0.009 | 4.419 | 0.1248645590393216 | 0.8880356574091361 | 326 | ||
| L20 | 137 | 2018-05-29T21:20:0+0000 | 31.5095238095239 | 28.61249999999993 | 82.8 | 0.01 | 3.978 | 0.20362285498323796 | 1.7913822744287753 | 326 | ||
| L21 | 45 | 2018-05-29T22:23:0+0000 | 31.73214285714281 | 30.06000000000005 | 0 | 0 | 3.968 | 0.283059959909195 | 1.3014315668926997 | 326 | ||
| L22 | 54 | 2018-05-29T23:15:0+0000 | 33.22380952380949 | 32.31333333333334 | 88.2 | 0.011 | 3.843 | 0.1939480284663809 | 0.9645904554616481 | 326 | ||
