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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR07 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR07 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 32°C for 35 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/914 | ||||||||
| OR | |||||||||
| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| N0 | 8.105 | 0 | 5.114 | 305 | ||||||||
| N1 | 19.5 | 2019-02-06T9:15:0+0000 | 0.21071428571431805 | 0.10033333333336891 | 0.545 | 6.58 | 7.362 | 0.315 | 5.103 | 305 | ||
| N2 | 14 | 2019-02-06T9:54:0+0000 | 0.46547619047622646 | 0.11766666666668257 | 0.9099999999999999 | 5.34 | 7.204 | 0.386 | 5.131 | 305 | ||
| N3 | 9.55 | 2019-02-06T10:38:0+0000 | 0.6464285714286033 | 0.026666666666545307 | 1.515 | 6.3 | 6.682 | 0.465 | 5.118 | 305 | ||
| N4 | 13 | 2019-02-06T11:24:0+0000 | 1.1750000000000054 | 0.6539999999999324 | 2.5700000000000003 | 5.460000000000001 | 5.73 | 0.54 | 5.124 | 305 | ||
| N5 | 10 | 2019-02-06T11:59:0+0000 | 1.7964285714286037 | 0.6374999999999575 | 4.33 | 3.92 | 4.27 | 0.655 | 5.187 | 305 | ||
| N6 | 10 | 2019-02-06T12:53:0+0000 | 3.4499999999999216 | 2.4408333333334475 | 8.350000000000001 | 1.29 | 1.457 | 0.543 | 5.161 | 305 | ||
| N7 | 11 | 2019-02-06T13:50:0+0000 | 5.41785714285711 | 5.818749999999984 | 14.000000000000002 | 0.019 | 0.303 | 5.141 | 305 | |||
| N8 | 9 | 2019-02-06T14:48:0+0000 | 7.639285714285721 | 6.915833333333232 | 20.9 | 0.003 | 0 | 4.988 | 305 | |||
| N9 | 9 | 2019-02-06T15:26:0+0000 | 9.857142857142883 | 8.49499999999992 | 26.6 | 0.013 | 0 | 4.847 | 305 | |||
| N10 | 7 | 2019-02-06T16:42:0+0000 | 15.671428571428464 | 13.749999999999929 | 40.60000000000001 | 0.006 | 0 | 4.471 | 305 | |||
| N11 | 7.5 | 2019-02-06T17:36:0+0000 | 19.33214285714294 | 15.426666666666478 | 47.8 | 0 | 4.303 | 305 | ||||
| N12 | 10 | 2019-02-06T18:36:0+0000 | 21.1678571428572 | 19.51500000000017 | 51 | 0 | 3.818 | 305 | ||||
| N13 | 9.5 | 2019-02-06T19:45:0+0000 | 23.35000000000008 | 21.910000000000096 | 55.400000000000006 | 0.036 | 305 | |||||
| N14 | 62 | 2019-02-06T21:10:0+0000 | 25.146428571428526 | 23.134999999999906 | 61.4 | 0.042 | 3.402 | 0.13577010383687738 | 0.6201070471162259 | 305 | ||
| N15 | 60 | 2019-02-06T22:4:0+0000 | 25.239285714285643 | 23.670000000000524 | 61.8 | 0.041 | 3.345 | 0.16669112253684473 | 0.15736313737996555 | 305 | ||
| N16 | 35.5 | 2019-02-06T23:30:0+0000 | 24.039285714285707 | 22.470000000000212 | 60.599999999999994 | 0 | 3.211 | 0.2213196259445807 | 0 | 305 | ||
| N17 | 35 | 2019-02-07T1:0:0+0000 | 25.0285714285714 | 22.06000000000019 | 62.400000000000006 | 0.024 | 3.067 | 0.20870591274598155 | 0 | 305 | ||
| N18 | 43 | 2019-02-07T3:0:0+0000 | 26.257142857142792 | 23.529999999999607 | 66.80000000000001 | 0 | 2.938 | 0.29225117102875736 | 0.8575812036962951 | 305 | ||
| N19 | 66 | 2019-02-07T7:20:0+0000 | 27.400000000000045 | 26.8250000000001 | 75.60000000000001 | 0 | 2.757 | 0.29581820189585994 | 0.5524701191937809 | 305 | ||
| N20 | 36 | 2019-02-07T9:0:0+0000 | 28.4607142857143 | 23.91000000000032 | 77.4 | 0.041 | 2.69 | 305 | ||||
| N21 | 6 | 2019-02-07T11:30:0+0000 | 28.682142857142896 | 27.121666666666933 | 79 | 0.019 | 2.602 | 0.5245997023311813 | 0.23358702379359084 | 305 | ||
| N22 | 32 | 2019-02-07T13:10:0+0000 | 28.78571428571429 | 29.750000000000387 | 76.4 | 0 | 2.503 | 0.4067689316090695 | 0.949724398352664 | 305 | ||
| N23 | 75 | 2019-02-07T15:57:0+0000 | 28.80357142857139 | 28.634999999999966 | 77.4 | 0.043 | 2.379 | 305 | ||||
| N24 | 47 | 2019-02-07T19:0:0+0000 | 29.799999999999986 | 30.41000000000027 | 81.39999999999999 | 0.025 | 2.224 | 0.3150083537231045 | 1.950824569091684 | 305 | ||
| N25 | 34 | 2019-02-07T22:0:0+0000 | 31.125000000000025 | 28.594999999999704 | 81.5 | 0.014 | 2.091 | 0.32115541825252036 | 2.363174222724295 | 305 | ||
| N26 | 37.5 | 2019-02-08T0:35:0+0000 | 30.882142857142874 | 30.671666666666653 | 81 | 0.015 | 1.982 | 0.395242188081173 | 2.8709226156234346 | 305 | ||
| N27 | 152 | 2019-02-08T8:11:0+0000 | 31.935714285714386 | 32.119999999999926 | 85 | 0 | 1.592 | 0.7119798644047858 | 5.054338263201621 | 305 | ||
