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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR08 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain E. coli NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/915 | ||||||||
| OR | |||||||||
| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| J0 | 9.091 | 5.332 | 305 | |||||||||
| J1 | 9 | 2019-02-19T8:37:0+0000 | 0.503571428571397 | 0.1803571428572935 | 0.59 | 8.129999999999999 | 8.209 | 0.305 | 5.339 | 305 | ||
| J2 | 11 | 2019-02-19T9:25:0+0000 | 0.6392857142856995 | 0.3392857142857694 | 1.1749999999999998 | 7.09 | 7.657 | 0.371 | 5.331 | 305 | ||
| J3 | 10 | 2019-02-19T10:28:0+0000 | 1.3357142857143132 | 0.8962500000000428 | 2.565 | 6.28 | 6.357 | 0.472 | 5.309 | 305 | ||
| J4 | 9.5 | 2019-02-19T11:39:0+0000 | 2.996428571428535 | 2.504651162790685 | 6.194999999999999 | 2.7600000000000002 | 3.055 | 0.535 | 5.306 | 305 | ||
| J5 | 11 | 2019-02-19T12:37:0+0000 | 5.678571428571439 | 4.4800000000000395 | 13 | 0.017 | 0.438 | 5.304 | 305 | |||
| J6 | 11 | 2019-02-19T13:35:0+0000 | 7.1428571428571495 | 6.3360000000000305 | 17.9 | 0.015 | 0.268 | 4.852 | 305 | |||
| J7 | 9 | 2019-02-19T14:27:0+0000 | 10.171428571428594 | 10.36999999999999 | 25.4 | 0.015 | 0.263 | 5.079 | 305 | |||
| J8 | 10.5 | 2019-02-19T15:32:0+0000 | 14.403571428571421 | 14.879999999999782 | 35.4 | 0.012 | 0.263 | 4.888 | 305 | |||
| J9 | 12 | 2019-02-19T16:15:0+0000 | 17.9821428571429 | 18.244999999999845 | 48.6 | 0.011 | 0.267 | 4.67 | 305 | |||
| J10 | 9 | 2019-02-19T16:52:0+0000 | 22.189285714285802 | 19.979999999999887 | 54.800000000000004 | 0.008 | 0.266 | 4.499 | 305 | |||
| J11 | 60 | 2019-02-19T17:40:0+0000 | 25.66428571428573 | 25.465000000000515 | 60.199999999999996 | 0.031 | 0.802 | 4.271 | 0.24265899421903328 | 0.680576835943135 | 305 | |
| J12 | 57 | 2019-02-19T18:45:0+0000 | 27.80357142857142 | 26.59500000000037 | 63.8 | 0.04 | 0.393 | 4.068 | 0 | 1.6670120626136486 | 305 | |
| J13 | 56 | 2019-02-19T20:11:0+0000 | 28.210714285714328 | 28.68499999999985 | 69.6 | 0.035 | 0.293 | 4.006 | 0.13138629327542012 | 2.564781934183747 | 305 | |
| J14 | 35 | 2019-02-19T21:17:0+0000 | 28.849999999999948 | 28.019999999999712 | 70.6 | 0.016 | 0.299 | 3.931 | 0.21138945930353545 | 1.6294732414740247 | 305 | |
| J15 | 58 | 2019-02-19T22:45:0+0000 | 29.8214285714287 | 28.955000000000286 | 72.4 | 0.005 | 0.305 | 3.741 | 0.18237874673346266 | 2.4363034747288514 | 305 | |
| J16 | 35 | 2019-02-20T1:28:0+0000 | 30.18571428571434 | 29.56499999999984 | 75.8 | 0.031 | 0.306 | 3.585 | 305 | |||
| J17 | 58 | 2019-02-20T7:1:0+0000 | 32.489285714285636 | 32.415000000000305 | 82.8 | 0 | 0.297 | 3.194 | 0.300323317660879 | 4.6187548238265625 | 305 | |
| J18 | 29 | 2019-02-20T8:40:0+0000 | 32.9321428571429 | 32.42999999999974 | 81.39999999999999 | 0 | 0.299 | 3.102 | 0.21723732798040024 | 2.996784896456177 | 305 | |
| J19 | 7 | 2019-02-20T10:10:0+0000 | 33.23571428571426 | 31.80000000000005 | 87.25 | 0 | 0.29 | 2.985 | 305 | |||
| J20 | 10 | 2019-02-20T11:38:0+0000 | 32.935714285714354 | 34.12000000000015 | 88.5 | 0.003 | 0.274 | 2.902 | 0.2741242571871842 | 2.8722170174310255 | 305 | |
| J21 | 52 | 2019-02-20T14:40:0+0000 | 34.92500000000002 | 34.98000000000046 | 87.99999999999999 | 0.018 | 2.754 | 305 | ||||
| J22 | 54 | 2019-02-20T17:40:0+0000 | 35.325000000000024 | 35.960000000000214 | 89.75 | 0.032 | 2.534 | 0.3605337329648725 | 0.759722521130665 | 305 | ||
| J23 | 41 | 2019-02-20T20:20:0+0000 | 36.71428571428564 | 36.244999999999635 | 95.25 | 0.012 | 0.32 | 2.379 | 0.5631123171455488 | 1.5889887976628019 | 305 | |
| J24 | 58 | 2019-02-20T23:2:0+0000 | 36.549999999999955 | 35.94000000000008 | 87.74999999999999 | 0.03 | 0.346 | 2.266 | 0.26272622290109077 | 2.985936302559418 | 305 | |
| J25 | 128 | 2019-02-21T7:2:0+0000 | 37.45357142857144 | 38.56499999999974 | 93.25 | 0.005 | 0.471 | 1.033 | 0.8144292264403359 | 4.452527353523323 | 305 | |
