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| Investigation | Study | Assay | Assigned to (Hub email ; separation) | Assigned to Institute or facility according to hub name (e.g. Wageningen University) | Input material | Output material | Protocol (URL) | |||||||||||||
| Understanding the extracellular environment in Aspergillus niger | ||||||||||||||||||||
| In silico prediction | ||||||||||||||||||||
| Obtain proteomes | A. niger strains CBS 513.88; ATCC 1015; A. oryzae RIB40; A. fumigatus AF293; A. nidulans FGSC A4 | |||||||||||||||||||
| Signal peptide predictions | A. niger strains CBS 513.88; ATCC 1015; A. oryzae RIB40; A. fumigatus AF293; A. nidulans FGSC A4 | |||||||||||||||||||
| Orthology identification | A. niger strains CBS 513.88; ATCC 1015; A. oryzae RIB40; A. fumigatus AF293; A. nidulans FGSC A4 | Orthology clusters | ||||||||||||||||||
| GPI Anchor predictions | Orthology clusters | GPI proteins | ||||||||||||||||||
| Culture experiments | ||||||||||||||||||||
| growth experiment | A. niger wild type N402 | condition table; samples | ||||||||||||||||||
| Liquid chromatography tandem mass spectrometric | samples | MS Spectra | ||||||||||||||||||
| validation of the secretome of A. niger | ||||||||||||||||||||
| MS analysis | MS Spectra; Proteome of A. niger strain CBS 513.8; Proteom of ATCC 1015 | Peptides; locus tags | ||||||||||||||||||
| Metabolic model analysis | ||||||||||||||||||||
| Obtain metabolic model | SBML model | |||||||||||||||||||
| Identify enzymes from specta | locus tags; SBML model | |||||||||||||||||||
