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Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR04 Channel 4: gas out - BR05 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
5L bioreactor of E. coli CH10-12 in a batch/fed-batch strategy at 37°C, induction promoted for 6h at 33°C MFCS BioPAT Sartorius