SEEK ID: https://ibisbahub.eu/people/215
Location:
France
ORCID:
https://orcid.org/0000-0001-8290-1635
Joined: 10th Apr 2025
Expertise: Not specified
Tools: Not specified
Related items
- Projects (1)
- Institutions (1)
- Investigations (0+1)
- Studies (4)
- Assays (9)
- Data files (0+4)
- Publications (2)
- Events (0+1)
- Documents (1)
- Observation Units (0+2)
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
Submitter: Susana Alonso Villela
Investigation: 1 hidden item
Snapshots: No snapshots
Submitter: Susana Alonso Villela
Investigation: 1 hidden item
Snapshots: No snapshots
Submitter: Susana Alonso Villela
Investigation: 1 hidden item
Assays: Fed-batch culture of CH10-12 strain, induction at 29°C, Fed-batch culture of CH10-12 strain, induction at 32°C, Fed-batch culture of NbF12-10 strain, induction at 29°C
Snapshots: No snapshots
Submitter: Susana Alonso Villela
Investigation: 1 hidden item
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C, Fed-batch culture of CH10-12 strain, induction at 30°C, Fed-batch culture of CH10-12 strain, induction at 33°C, Fed-batch culture of CH10-12 strain, induction at 37°C
Snapshots: No snapshots
Submitter: Susana Alonso Villela
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: 1 hidden item
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Ghezal-Kraïem, Balkiss Bouhaouala-Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Feb 2021
Publication Type: Journal
DOI: 10.1007/s00253-021-11093-5
Citation: Appl Microbiol Biotechnol 105(3):1017-1030
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Kraïem, Balkiss Bouhaouala‐Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Jun 2020
Publication Type: Journal
DOI: 10.1002/mbo3.1027
Citation: MicrobiologyOpen 9(6):1175-1182
Procedure followed for the data treatment and analysis of 5L bioreactor cultures
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Investigations: 1 hidden item
Studies: Characterization of E. coli WK6 strain under ae..., Characterization of E. coli WK6 strain under in..., Recombinant nanobody CH10-12 and NbF12-10 produ..., Recombinant nanobody CH10-12 production at diff...
Assays: Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of CH10-12 strain, induction ..., Fed-batch culture of NbF12-10 strain, induction..., Fed-batch culture of WK6 strain, induction at 29°C, Fed-batch culture of WK6 strain, without induction