Conjugation procedure of pK18mobSacB plasmid from E. coli S17pir strain to P. putida KT2440 strain

This protocol describes the procedure of conjugation of pK18mobSacB suicide plasmid containing fragments UP and DOWN that delimit the recombination region from E. coli S17λpir to P. putida cells.

SEEK ID: https://ibisbahub.eu/sops/120?version=2

Filename: Conjugation procedure of pK18mobSacB plasmid from E. coli S17pir strain to P. putida KT2440 strain.docx 

Format: Word document

Size: 29.3 KB

help Creators and Submitter
Creator
Additional credit

Laura de Eugenio, Beatriz Galán

Submitter
License
Creative Commons Attribution 4.0 International
Activity

Views: 2617   Downloads: 1

Created: 4th Aug 2021 at 16:29

Last updated: 4th Aug 2021 at 16:30

help Tags

This item has not yet been tagged.

help Attributions

None

Version History

Version 2 (latest) Created 4th Aug 2021 at 16:30 by Felipe de Salas

No revision comments

Version 1 (earliest) Created 4th Aug 2021 at 16:29 by Felipe de Salas

No revision comments

Powered by
 (v.1.16.0-pre)
About IBISBA | About IBISBA Knowledge Hub | Funding and Programmes | Credits | Terms & Conditions
Copyright © 2008 - 2024 The University of Manchester and HITS gGmbH
IBISBA is a pan-European research infrastructure that is currently funded by the EU Horizon 2020 projects
IBISBA 1.0 (grant agreement number 730976) and PREP-IBISBA (grant agreement number 871118).
Registering data or other knowledge assets on this platform is the sole responsibility of Users.
IBISBA cannot be held responsible for misuse or misappropriation of data and assets belonging to a Third Party..