IBISBA Library

A description of the content and format of SOPs for IBISBA

2nd IBISBA Webinar on Protein Engineering and Biocatalysis

Thu, Mar 26, 2020 9:00 AM - 11:00 AM GMT

This second course is about Protein Engineering and Biocatalysis and will be focused on 4 main topics:

How computational protein design help to predict sequences for new and improved functions
How their catalytic activity can be measured through high-throughput enzyme screening
How engineered proteins/enzymes can be produced at large scale using eukaryotic microbes
How enzymes can be used in
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IBISBA 1.0 First Webinar Series

How to accelerate your research or technology and get subsidised access to top research infrastructure

The first webinar out of a series of four will illustrate:

1) What IBISBA 1.0 is
2) How you can gain Transnational Access to this Pan-European Research Infrastructure for Industrial Biotechnology
3) How to create metabolic models at genomic scale and develop Synthetic Biology tools

This is the agenda:

A vision paper describing the founding principles behind IBISBA

This DMP relates to the IBISBA 1.0 project

A set of agreed identification schemes to be used within the IBISBA project to reference resources

Formats agreed to be used in IBISBA

Given a collection of SBML files with the heterologous pathways, calculate the FBA and the Gibbs free energy of the pathways.

Given one or a collection of heterolgous pathways in SBML files, generate the SBOL for the constructs and the assembly protocols to build them

Perform retrosynthesis using RetroPath2.0 and convert the heterologous pathways to SBML files

Galaxy workflow that predicts heterologous pathways for the production of a compound of interest in an organism of interest

The ligase chain reaction (LCR) is a method of DNA amplification that differs from PCR in that it involves a thermostable ligase to join two probes or other molecules together which can then be amplified by standard PCR cycling.

This tool uses a multi-objective algorithm to predict the best synthesis and assembling strategy for the previously designed plasmid using either Golden gate assembly, or Gibson assembly, or a mix of both. Given a set of designs (one design is a construct name and list of its parts), it finds a valid and efficient assembly plan to build all the designs. The designs and sequences of parts are provided as an SBOL file (see test_input.xml for an example).

This tool allows for the design and optimization of novel, reusable synthetic biology parts. For each enzyme predicted, it recovers the DNA sequence from the UNIPROT ID, runs a codon optimization protocol, and adds various strength calculated RBS (ribosome binding site) to it.

This tool takes as input a TAR collection of rpSBML files, that contain for each heterologous reaction a ranked list of UniProt identifiers for the sequences, and the list of links to the DNA registry SynBioHub for the desired vector backbones, resistance cassette, and promoters (generated using the tool "OptDoE Parts Reference Generator"). An optimal design of experiments is performed by using OptBioDes based on logistic regression analysis with an assumed linear model for the response. The
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Scans for the reaction rules and makes a REST request to Selenzyme and finds the enzymatic sequences (through Uniprot ID's) of all the reactions in heterologous pathways of rpSBML files.