SOPs

What is a SOP?
23 SOPs visible to you, out of a total of 49
Acetate kinase protein activity assay
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Agarose gel electrophoresis of DNA fragments
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

cDNA synthesis
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Cleaning of electroporation cuvettes
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Colony PCR of S. cerevisiae, P. putida, E. coli
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Cultivation of P. putida (and E. coli) in rich-, minimal-, and selective medium
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Design of spacers and cloning of them in pSEVA231-CRISPR
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Determination of cell dry weight
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

DNA and RNA concentration measurement
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Enzymatic actyl-CoA determination
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Genomic DNA isolation
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Golden gate cloning
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Gram staining of bacteria
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Inoculating and sampling anaerobic cultures and CFU determination
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Live/dead staining of bacteria
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Making a growth curve of a bacterial or yeast strain
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Making electro-competent E. coli cells and transformation of them
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Marker-less multiple gene deletions in P. putida KT2440
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

MASC-PCR
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Mix&Go competent cells and transformation of E. coli
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Plasmid isolation from E. coli and P. putida using the Thermo Scientific GeneJET Plasmid Miniprep Kit
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Restriction / digestion of DNA
No description specified

Creator: Rita Volkers

Submitter: Rita Volkers

Transformation of Pseudomonas putida by electroporation

This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.

All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...

Creator: Rita Volkers

Submitter: Rita Volkers

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