SEEK ID: https://ibisbahub.eu/studies/77
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Projects: Muconic Acid Production
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Created: 11th Jan 2021 at 13:21
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IBISBA offers access to best-in-class facilities and know-how. Clients from academia, SMEs and big industry can find tailored solutions for their R&D needs.
Using a H2020 funding framework, IBISBA currently offers limited opportunities to researchers to obtain subsidised access to IBISBA specialised research structures and facilities, including contribution to user's travel and subsistence costs. Open Calls are launched regularly, inviting researchers from all sectors (academia and industry) ...
Projects: TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, FLOX: FLavin-containing OXidoreductases, PlanOvac, HIGHFLUX, SporeDel, Yeast Glycerol, Galambr, SULFOCEL, Muconic Acid Production, Promoters 4 Pichia, Evocolour - The evolution and genetics of Flavobacteria forming structurally coloured colonies for sustainable biomaterials, Extreme - Genomic approach for extremozymes screening of thermophilic/anaerobic Caldicoprobacter algerensis strain, HMOzyme, PILOT-MP - Pilot scale process development for microbial proteins, UMB - Upscaling and optimisation of microalgae production for bivalves hatchery, Biocare, MEOX, Coculture, YarroFuels, PlasticPutida, Lipro-alga, Cyanofcs, ENGINEER4ARGININE, Upscale of the biocatalytic transformation of Free Fatty Acids into high-value products (BIOFFA), B-PAC, BIOFFA, GLYCOMICS, KorallionBio, MAROX, PROTEIFROM, SUPI
Construction of a P. putida KT2440 producing cis,cis-muconate from glucose, benzoate, or p-coumarate
Programme: TransNational Access
Public web page: Not specified
Organisms: Pseudomonas putida
This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.
All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...
Creator: Rita Volkers
Submitter: Rita Volkers
Investigations: 2 hidden items
Studies: Transformation of production host and 1 hidden item
Assays: 2 hidden items