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SEEK ID: https://hub.ibisba.eu/people/91
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The collection of protocols that have not yet been curated by IBISBA WP6
Programme: IBISBA
Public web page: http://www.ibisba.eu
Construction of a P. putida KT2440 producing cis,cis-muconate from glucose, benzoate, or p-coumarate
Organisms: Pseudomonas putida
This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.
All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial
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Creator: Rita Volkers
Contributor: Rita Volkers
Creator: Rita Volkers
Contributor: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Contributor: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Contributor: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Creator: Rita Volkers
Contributor: Rita Volkers
Investigations: No Investigations
Studies: No Studies
Assays: No Assays