SOPs
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E. coli6
P. putida6
DNA3
transformation3
competent2
electro competent2
electroporation2
PCR2
RNA2
staining2
Acetata kinase1
Acetyl-CoA1
agarose1
anaerobic1
assay1
bacteria1
cDNA1
cdw1
cell dry weight1
CFU1
cleaning1
Cloning1
Colony1
Concentration1
CRISPR1
cultivation1
cuvette1
deletion1
design1
digestion1
electrophoresis1
gDNA1
gel1
gene1
genomic DNA1
Golden gate1
Gram1
gram staining1
growth curve1
inoculating1
isolation1
live/dead1
MASC1
medium1
minimal1
miniprep1
mix & go1
multiple1
Plasmid isolation1
pSEVA2311
restriction1
rich1
S. cerevisiae1
sampling1
selective1
Spacer1
synthesis1
yeast1
More...
Submitter
Rita Volkers23
Project
Uncurated Protocols Library23
Programme
IBISBA23
Creator
Rita Volkers23
Assay type
Experimental Assay Type2
Technology type
Technology Type2
23
SOPs visible to you, out of a total of 89
This is a Protocol for transforming plasmid DNA into P. putida cells by electroporation. This is a fast and easy-to-use method of introducing plasmid DNA in P. putida cells with high transformation efficiency. This Protocol is adapted from [1] and encompasses the steps for the preparation of competent cells and transformation of plasmid DNA by electroporation.
All Inputs of the Protocol, listed in the section 4.1., can be acquired either from other IBISBA Protocols or purchased from commercial ...
Creator: Rita Volkers
Submitter: Rita Volkers