TransNational Access

Overview
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IBISBA offers access to best-in-class facilities and know-how. Clients from academia, SMEs and big industry can find tailored solutions for their R&D needs.

Using a H2020 funding framework, IBISBA currently offers limited opportunities to researchers to obtain subsidised access to IBISBA specialised research structures and facilities, including contribution to user's travel and subsistence costs. Open Calls are launched regularly, inviting researchers from all sectors (academia and industry) to apply.

Web page: https://www.ibisba.eu/Our-Offer/Subsidised-Access

Funding codes:

European Union Horizon 2020/730976

Funding details:

No funding details specified

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Showing 5 out of a possible 55 items Advanced People list for this Programme with search and filtering ...

IBISBA Admin

Projects: Uncurated Protocols Library, TRANSCRIPTPROM - Integrative and comparative analyses of response of Pichia pastoris, FLOX: FLavin-containing OXidoreductases, PlanOvac, Muconic Acid Production, HIGHFLUX, Promoters 4 Pichia, An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data, Template library, Evocolour - The evolution and genetics of Flavobacteria forming structurally coloured colonies for sustainable biomaterials, Extreme - Genomic approach for extremozymes screening of thermophilic/anaerobic Caldicoprobacter algerensis strain, HMOzyme, PILOT-MP - Pilot scale process development for microbial proteins, UMB - Upscaling and optimisation of microalgae production for bivalves hatchery, Public announcements and documents, IBISBA Library, Biocare, MEOX, Coculture, YarroFuels, PlasticPutida, Lipro-alga, Curated Protocols Library, IBISBA 1.0 deliverables, PREP-IBISBA deliverables, Cyanofcs, Example testing project

Institutions: Administration, UNIMAN - The University of Manchester

Gregorio Álvaro

Projects: Uncurated Protocols Library, MEOX

Institutions: UAB - Universitat Autònoma de Barcelona

https://orcid.org/0000-0002-2924-8902

Expertise: Biotechnology, Downstream Processing, Biocatalysis, Multienzymatic Cascade reactions, Cofactor regeneration, enzymatic reactors design, Biocatalytic processes, Biocatalysts Immobilization

Tools: Biocatalysts Immobilization, Enzymatic reaction courses, Enzymatic Activity Assays, Enzymatic Stability Assays

Associated Professor Department of Chemical, Biological and Environmental Engineering Engineering School Universitat Autònoma de Barcelona (UAB)

Munazah Andrabi

Projects: Uncurated Protocols Library, FLOX: FLavin-containing OXidoreductases, PlanOvac, HIGHFLUX, SporeDel, Yeast Glycerol, Galambr, SULFOCEL, An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data, Template library, Muconic Acid Production in Yeast, IBISBA 1.0 deliverables, PREP-IBISBA deliverables, Biocare, test, DBTL-P, Upscale of the biocatalytic transformation of Free Fatty Acids into high-value products (BIOFFA), B-PAC, BIOFFA, GLYCOMICS, KorallionBio, MAROX, PROTEIFROM, SUPI

Institutions: UNIMAN - The University of Manchester, UAB - Universitat Autònoma de Barcelona, CNR - Consiglio Nazionale delle Ricerche, INSAT - Institut National des Science Appliquees de Toulouse, VTT - Teknologian tutkimuskeskus VTT Oy, VITO - Vlaamse Instelling voor Technologisch Onderzoek N.V.

Jorge Barriuso

Projects: Uncurated Protocols Library, Muconic Acid Production, Biocare, YarroFuels, PlasticPutida, Muconic Acid Production in Yeast, Curated Protocols Library, MEOX, Extreme - Genomic approach for extremozymes screening of thermophilic/anaerobic Caldicoprobacter algerensis strain

Institutions: CSIC - Agencia Estatal Consejo Superior de Investigaciones Cientificas

Showing 5 out of a possible 31 items Advanced Projects list for this Programme with search and filtering ...

Coculture

Co-Culture for improved lignocellulose fractionation and elicitation of enzymatic activies - Omics on co-culture of trichoderma reesei or Phanerochaete chrysosporium

Programme: TransNational Access

Public web page: Not specified

Organisms: Not specified

HIGHFLUX

High-resolutions fluxomics of industrial cyanobacteria

Programme: TransNational Access

Public web page: Not specified

Organisms: Not specified

FLOX: FLavin-containing OXidoreductases

Optimisation of production of flavin-containing oxidoreductases for industrial application

Programme: TransNational Access

Public web page: Not specified

Organisms: Not specified

PlanOvac

Plant-based bio-encapsulation strategies for oral veterinary vaccines

Programme: TransNational Access

Public web page: Not specified

Organisms: Not specified

SULFOCEL

Novel (ligno)cellulose degrading enzyme for expression in the thermoacidofilic Archaeon Sulfolobus acidocaldarius

Programme: TransNational Access

Public web page: Not specified

Organisms: Sulfolobus acidocaldarius

Showing 5 out of a possible 19 items Advanced Institutions list for this Programme with search and filtering ...

Activatec

Country: United Kingdom

City: Nottingham

Web page: https://www.activatec-bi.com/

Administration

Country: France

City: Not specified

Web page: Not specified

AINIA

Country: Spain

City: Valencia

Web page: https://www.ainia.es/

CEA - Commissariat a l'Energie Atomique et aux Energies Alternatives

Country: France

City: Paris

Web page: http://www.cea.fr/

CNR - Consiglio Nazionale delle Ricerche

Country: Italy

City: Rome

Web page: https://www.cnr.it

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21 hidden items

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Transformation of production host

Muconic Acid Production

No description specified

Submitter: Luis Garcia Morales

Investigation: 1 hidden item

Assays: No Assays

Snapshots: No snapshots

Created: 11th Jan 2021 at 13:21

Assemble parts

Muconic Acid Production

No description specified

Submitter: Luis Garcia Morales

Investigation: 1 hidden item

Assays: No Assays

Snapshots: No snapshots

Created: 11th Jan 2021 at 13:20

35 hidden items

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154 hidden items

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Results file

Promoters 4 Pichia

Summay of results form fermentations

Creator: Joan Albiol

Submitter: Joan Albiol

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 5th Jun 2020 at 08:11

50 hidden items

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10 hidden items

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Metagenomic and culturomic DNA and RNA extractions

SULFOCEL

No description specified

Creator: Beatrice Cobucci-Ponzano

Submitter: Beatrice Cobucci-Ponzano

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 13th Apr 2022 at 12:39

Lignocellulosic biomass pretreatment protocol

SULFOCEL

No description specified

Creator: Beatrice Cobucci-Ponzano

Submitter: Beatrice Cobucci-Ponzano

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 13th Apr 2022 at 12:36

In vitro enrichments of microbial environmental sample from high-temperature environments

SULFOCEL

No description specified

Creator: Beatrice Cobucci-Ponzano

Submitter: Beatrice Cobucci-Ponzano

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 13th Apr 2022 at 12:33

Infiltration of N. benthamiana leaves with Agrobacterium tumefaciens

PlanOvac

Leaf agroinfiltration is a method used in plant biology and biotechnology to induce transient expression of genes in leaf tissue, in order to produce a desired protein. In the method, a suspension of Agrobacterium tumefaciens, which contains a plasmid with the gene of interest, is introduced into a plant leaf by direct injection, whereafter the bacteria transfer the desired gene into the plant cells via transfer of T-DNA. The main benefit of agroinfiltration, when compared to the more traditional ...

Creators: Emanuela Pedrazzini, Emanuela Pedrazzini

Submitter: Emanuela Pedrazzini

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 2nd Feb 2021 at 15:24

30 hidden items

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GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways

HIGHFLUX

Abstract (Expand)

Abstract Background Cyanobacteria receive huge interest as green catalysts. While exploiting energy from sunlight, they co-utilize sugar and CO 2 . This photomixotrophic mode enables fast growth and …ze sugar and CO 2 . This photomixotrophic mode enables fast growth and high cell densities, opening perspectives for sustainable biomanufacturing. The model cyanobacterium Synechocystis sp. PCC 6803 possesses a complex architecture of glycolytic routes for glucose breakdown that are intertwined with the CO 2 -fixing Calvin-Benson-Bassham (CBB) cycle. To date, the contribution of these pathways to photomixotrophic metabolism has remained unclear. Results Here, we developed a comprehensive approach for 13 C metabolic flux analysis of Synechocystis sp. PCC 6803 during steady state photomixotrophic growth. Under these conditions, the Entner-Doudoroff (ED) and phosphoketolase (PK) pathways were found inactive but the microbe used the phosphoglucoisomerase (PGI) (63.1%) and the oxidative pentose phosphate pathway (OPP) shunts (9.3%) to fuel the CBB cycle. Mutants that lacked the ED pathway, the PK pathway, or phosphofructokinases were not affected in growth under metabolic steady-state. An ED pathway-deficient mutant ( Δeda ) exhibited an enhanced CBB cycle flux and increased glycogen formation, while the OPP shunt was almost inactive (1.3%). Under fluctuating light, ∆eda showed a growth defect, different to wild type and the other deletion strains. Conclusions The developed approach, based on parallel 13 C tracer studies with GC–MS analysis of amino acids, sugars, and sugar derivatives, optionally adding NMR data from amino acids, is valuable to study fluxes in photomixotrophic microbes to detail. In photomixotrophic cells, PGI and OPP form glycolytic shunts that merge at switch points and result in synergistic fueling of the CBB cycle for maximized CO 2 fixation. However, redirected fluxes in an ED shunt-deficient mutant and the impossibility to delete this shunt in a GAPDH2 knockout mutant, indicate that either minor fluxes (below the resolution limit of 13 C flux analysis) might exist that could provide catalytic amounts of regulatory intermediates or alternatively, that EDA possesses additional so far unknown functions. These ideas require further experiments.

Authors: Dennis Schulze, Michael Kohlstedt, Judith Becker, Edern Cahoreau, Lindsay Peyriga, Alexander Makowka, Sarah Hildebrandt, Kirstin Gutekunst, Jean-Charles Portais, Christoph Wittmann

Date Published: 1st Dec 2022

Publication Type: Journal

DOI: 10.1186/s12934-022-01790-9

Citation: Microb Cell Fact 21(1),69

Created: 30th Nov 2022 at 13:25, Last updated: 30th Nov 2022 at 13:25

Cell Biology Methods to Study Recombinant Proteins in Seeds

PlanOvac

Abstract

Not specified

Authors: Machtelt Braaksma, Elsa Arcalís, Elena S Martens-Uzunova, Emanuela Pedrazzini, Peter J Punt, Ulrike Hörmann-Dietrich, Peter J Schaap, Alessandro Vitale, Eva Stoger