Culture BR05 - induction phase

Excel file containing the data anlysis of the BR05 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Samples taken in the 5L bioreactor BR05 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 37°C for 6 h

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR04 Channel 4: gas out - BR05 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 37°C for 6h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 37°C for 6h