Industrial-scale penicillin simulation

Industrial-scale penicillin simulation

Industrial-scale penicillin simulation (IndPenSim) is a first principles mathematical model of a 100,000 Litre fermentation of an industrial strain of Penicillium chrysogenum validated using historical data collected from an industrial-scale penicillin fermentation process. Adapting a previously published structured model to describe penicillin fermentation and extending the model to include the main environmental effects of dissolved oxygen, viscosity, temperature, pH, and dissolved carbon dioxide. In addition the effects of nitrogen and phenylacetic acid concentrations on the biomass and penicillin production rates were also included. The manipulated variables recorded during each batch were used as inputs to the simulator and the predicted outputs were then compared with the on-line and off-line measurements recorded in the real process. Subsequently the simulation was extended to include the addition of a simulated Raman spectroscopy device with the purpose of this addition for the developing, evaluating and implementation of advanced and innovative control solutions applicable to biotechnology facilities.

As part of the Bioindustry4.0 project, the 100 batches of simulated data from IndPenSim have been used as a test dataset for the development of a number of tools and services given their scope and variability (e.g., faults, automated control, etc.).

References

• Goldrick, S., Ştefan, A., Lovett, D., Montague, G., and Lennox, B., 2015. The development of an industrial-scale fed-batch fermentation simulation, Journal of Biotechnology, Volume 193, Pages 70-82, DOI: 10.1016/j.jbiotec.2014.10.029, https://doi.org/10.1016/j.jbiotec.2014.10.029

• Goldrick, S., Duran-Villalobos, C. A., Jankauskas, K., Lovett, D., Farid, S. S., and Lennox, B., 2019. Modern day monitoring and control challenges outlined on an industrial-scale benchmark fermentation process, Computers and Chemical Engineering Journal Volume 130, 106471, DOI: 10.1016/j.compchemeng.2019.05.037, https://doi.org/10.1016/j.compchemeng.2019.05.037

URL

• https://nl.mathworks.com/matlabcentral/fileexchange/49041-industrial-scale-penicillin-simulationv2
• http://www.industrialpenicillinsimulation.com/
• https://github.com/StephenGoldie/indpensim-notebook