Recombinant nanobody CH10-12 production at different induction temperatures and duration

Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...

In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...

5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 37°C for 6 h

Excel file containing the data anlysis of the BR02 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR04 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR05 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Samples taken in the 5L bioreactor BR02 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h

Procedure followed for the data treatment and analysis of 5L bioreactor cultures

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR04 Channel 4: gas out - BR05 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

5L bioreactor of E. coli CH10-12 in a batch/fed-batch strategy at 37°C, induction promoted for 6h at 37°C MFCS BioPAT Sartorius

5L bioreactor of E. coli CH10-12 in a batch/fed-batch strategy at 37°C, induction promoted for 6h at 33°C MFCS BioPAT Sartorius

5L bioreactor of E. coli CH10-12 in a batch/fed-batch strategy at 37°C, induction promoted for 10h at 30°C MFCS BioPAT Sartorius