RWTH Aachen University

Real-time online monitoring of bioprocesses

Industrial biotechnology (IB) has the potential to create new markets while protecting the environment. With this in mind, the EU-funded BIOINDUSTRY 4.0 project will pave the way for IB to become a major manufacturing technology. Supporting the digitalisation of IB, the project will create new services delivered by European research infrastructure. These will address several challenges, focusing on the acceleration of bioprocess development pipelines. ...

This programme is for making IBISBA documents available to the public.

The fermentations were carried out at Vito utilizing the experience of VTT. The fermentations were monitored by both Timegate and Kaiser Raman spectrometers. The substrates used were xylose and xylitol and the product xylitol. Dissolved oxygen, off-gas analysis were also carried out. Offline samples were taken for further analysis. Further pure-component and mixture samples of the used components in the expected concentration ranges were prepared and measured by both devices and serve as calibration ...

This project make available the deliverables of the PREP-IBISBA project

This project collects together the IBISBA 1.0 deliverables

Measurements using Ramen spectroscopy can be perturbed by ‘background’ fluorescence during fermentation originating from the organism, the feedstock, and/or the medium. Data from the ‘Ethanol Production’ use case involving an industrial strain of the yeast S. cerevisiae (RWTH) will investigate the various sources of fluorescence and their effects on fluorescence at the end of fermentation. New and collated datasets will be useful in the production, refinement, and verification of statistical, ...