Fed-batch culture of E. coli strains in microbioreactors
Culture of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2017) at 28°C in a fed-batch microbioreactor setting.
SEEK ID: https://ibisbahub.eu/assays/918
Experimental assay
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
Investigation: Scale-down effects of the nanobody producing E. coli strains
Study: Characterization of nanobody-producer strains in microbioreactors
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: Escherichia coli
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Created: 20th Oct 2025 at 14:31
Last updated: 23rd Oct 2025 at 07:41
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Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Institutions: INSAT - Institut National des Science Appliquees de Toulouse, INRAE
https://orcid.org/0000-0001-8290-1635
Bioprocess engineer with extensive expertise in bioreactor process design, fermentation technologies, and protein production. Skilled in scaling up bioprocesses, experimental design, and process optimization.
Real-time online monitoring of bioprocesses
Industrial biotechnology (IB) has the potential to create new markets while protecting the environment. With this in mind, the EU-funded BIOINDUSTRY 4.0 project will pave the way for IB to become a major manufacturing technology. Supporting the digitalisation of IB, the project will create new services delivered by European research infrastructure. These will address several challenges, focusing on the acceleration of bioprocess development pipelines. ...
Projects: Industrial-scale penicillin simulation, Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment, Bioindustry4.0 Work Package 7: Tools for high-quality datasets, requisite for data-driven methods and services
Web page: https://www.bioindustry4.eu
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
We studied the production of scorpion antivenom nanobodies in high cell density microbioreactors. The BiolectorXT (Beckman) was used to screen different production strategies within the framework of a machine-learning model optimization.
The screening of E. coli strains WK6, CH10-12 (2017), CH10-12 (2023), NbF12-10 (2017), and NbF12-10 (2023) was made in a BioLectorXT (Beckman) setting
Submitter: Susana Alonso Villela
Investigation: Scale-down effects of the nanobody producing E....
Assays: Fed-batch culture of E. coli strains in microbioreactors
32 fed-batch cultures at 28°C
Creators: Susana Alonso Villela, Cesar ACEVES LARA, Juan Camilo Acosta Pavas
Submitter: Susana Alonso Villela
Relationship type: Not specified
