5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR09 samples, BioPAT MFCS data for BR09 - part 1, BioPAT MFCS data for BR09 - part 2, Data analysis of the BR09 culture, Gas analysis data for BR08 and BR09
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR08 samples, BioPAT MFCS data for BR08 - part 1, BioPAT MFCS data for BR08 - part 2, Data analysis of the BR08 culture, Gas analysis data for BR08 and BR09
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR07 samples, BioPAT MFCS data for BR07 - part 1, BioPAT MFCS data for BR07 - part 2, Data analysis of the BR07 culture, Gas analysis data for BR06 and BR07
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR06 samples, BioPAT MFCS data for BR06 - part 1, BioPAT MFCS data for BR06 - part 2, Data analysis of the BR06 culture, Gas analysis data for BR06 and BR07
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 37°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR05 samples, BioPAT MFCS data for BR05 - part 1, BioPAT MFCS data for BR05 - part 2, Data analysis of the BR05 culture, Gas analysis data for BR04 and BR05
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR04 samples, BioPAT MFCS data for BR04 - part 1, BioPAT MFCS data for BR04 - part 2, Data analysis of the BR04 culture, Gas analysis data for BR04 and BR05
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR03 samples, BioPAT MFCS data for BR03 - part 1, BioPAT MFCS data for BR03 - part 2, Data analysis of the BR03 culture, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR02 samples, BioPAT MFCS data for BR02 - part 1, BioPAT MFCS data for BR02 - part 2, Data analysis of the BR02 culture, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
Excel file containing the data anlysis of the BR09 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR08 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR07 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR06 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR05 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR04 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Excel file containing the data anlysis of the BR02 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR09 in the culture of _E. coli _WK6 in a batch/fed-batch strategy at 37°C. Induction simulation with 1 mM IPTG when biomass reached 25 g cdw/L and at 29°C for 28 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain _E. coli _NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR07 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 32°C for 35 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR06 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 38 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR05 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 37°C for 6 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR04 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 33°C for 6 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain_ E. coli_ CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR02 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Kraïem, Balkiss Bouhaouala‐Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Jun 2020
Publication Type: Journal
DOI: 10.1002/mbo3.1027
Citation: MicrobiologyOpen 9(6):1175-1182