Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR09 samples, BioPAT MFCS data for BR09 - part 1, BioPAT MFCS data for BR09 - part 2, Data analysis of the BR09 culture, Gas analysis data for BR08 and BR09
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR08 samples, BioPAT MFCS data for BR08 - part 1, BioPAT MFCS data for BR08 - part 2, Data analysis of the BR08 culture, Gas analysis data for BR08 and BR09
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR07 samples, BioPAT MFCS data for BR07 - part 1, BioPAT MFCS data for BR07 - part 2, Data analysis of the BR07 culture, Gas analysis data for BR06 and BR07
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR06 samples, BioPAT MFCS data for BR06 - part 1, BioPAT MFCS data for BR06 - part 2, Data analysis of the BR06 culture, Gas analysis data for BR06 and BR07
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR04 samples, BioPAT MFCS data for BR04 - part 1, BioPAT MFCS data for BR04 - part 2, Data analysis of the BR04 culture, Gas analysis data for BR04 and BR05
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR02 samples, BioPAT MFCS data for BR02 - part 1, BioPAT MFCS data for BR02 - part 2, Data analysis of the BR02 culture, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
Excel file containing the data anlysis of the BR02 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR09 in the culture of _E. coli _WK6 in a batch/fed-batch strategy at 37°C. Induction simulation with 1 mM IPTG when biomass reached 25 g cdw/L and at 29°C for 28 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain _E. coli _NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR07 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 32°C for 35 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR06 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 38 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR05 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 37°C for 6 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR04 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 33°C for 6 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain_ E. coli_ CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR02 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Ghezal-Kraïem, Balkiss Bouhaouala-Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Feb 2021
Publication Type: Journal
DOI: 10.1007/s00253-021-11093-5
Citation: Appl Microbiol Biotechnol 105(3):1017-1030