5L bioreactor culture of _E. coli _CH10-12 at 37°C in batch phase. Caracterization of biomass growth
SEEK ID: https://ibisbahub.eu/events/10
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
16th May 2018 at 05:28 (16th May 2018 at 07:28 (Europe/Paris))
10th Jun 2025 at 17:50 (10th Jun 2025 at 19:50 (Europe/Paris))
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Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain_ E. coli_ CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela