Fed-batch culture of CH10-12 strain, induction at 30°C - BR03
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h
SEEK ID: https://ibisbahub.eu/assays/910
Experimental assay
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
Investigation: Effect of the induction temperature in nanobody producing E. coli strains
Study: Recombinant nanobody CH10-12 production at different induction temperatures and duration
Assay position: 2
Assay type: Cultivation Experiment
Technology type: Fed-batch
Organisms: Escherichia coli
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Created: 18th Apr 2025 at 08:30
Last updated: 13th Jun 2025 at 10:49
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Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment
Institutions: INSAT - Institut National des Science Appliquees de Toulouse, INRAE
https://orcid.org/0000-0001-8290-1635
Bioprocess engineer with extensive expertise in bioreactor process design, fermentation technologies, and protein production. Skilled in scaling up bioprocesses, experimental design, and process optimization.
Real-time online monitoring of bioprocesses
Industrial biotechnology (IB) has the potential to create new markets while protecting the environment. With this in mind, the EU-funded BIOINDUSTRY 4.0 project will pave the way for IB to become a major manufacturing technology. Supporting the digitalisation of IB, the project will create new services delivered by European research infrastructure. These will address several challenges, focusing on the acceleration of bioprocess development pipelines. ...
Projects: Industrial-scale penicillin simulation, Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment, Bioindustry4.0 Work Package 7: Tools for high-quality datasets, requisite for data-driven methods and services
Web page: https://www.bioindustry4.eu
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...
Submitter: Susana Alonso Villela
Studies: Characterization of E. coli WK6 strain under aerobic conditions, Characterization of E. coli WK6 strain under induction conditions, Recombinant nanobody CH10-12 and NbF12-10 production at low induction te..., Recombinant nanobody CH10-12 production at different induction temperatu...
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C - BR02, Fed-batch culture of CH10-12 strain, induction at 29°C - BR06, Fed-batch culture of CH10-12 strain, induction at 30°C - BR03, Fed-batch culture of CH10-12 strain, induction at 32°C - BR07, Fed-batch culture of CH10-12 strain, induction at 33°C - BR04, Fed-batch culture of CH10-12 strain, induction at 37°C - BR05, Fed-batch culture of NbF12-10 strain, induction at 29°C - BR08, Fed-batch culture of WK6 strain, induction at 29°C - BR09, Fed-batch culture of WK6 strain, without induction - BR01
Submitter: Susana Alonso Villela
Investigation: Effect of the induction temperature in nanobody...
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C - BR02, Fed-batch culture of CH10-12 strain, induction at 30°C - BR03, Fed-batch culture of CH10-12 strain, induction at 33°C - BR04, Fed-batch culture of CH10-12 strain, induction at 37°C - BR05
Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain_ E. coli_ CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h
Procedure followed for the data treatment and analysis of 5L bioreactor cultures
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
