Fed-batch culture of NbF12-10 strain, induction at 29°C
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
SEEK ID: https://ibisbahub.eu/assays/915
Experimental assay
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
Investigation: hidden item
Assay position: 3
Assay type: Cultivation Experiment
Technology type: Fed-batch
Organisms: Escherichia coli
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Created: 18th Apr 2025 at 08:51
Last updated: 18th Apr 2025 at 13:07
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https://orcid.org/0000-0001-8290-1635 
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Submitter: Susana Alonso Villela
Investigation: 1 hidden item
Assays: Fed-batch culture of CH10-12 strain, induction at 29°C, Fed-batch culture of CH10-12 strain, induction at 32°C, Fed-batch culture of NbF12-10 strain, induction at 29°C
Procedure followed for the data treatment and analysis of 5L bioreactor cultures
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
