An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data

The ecological niche occupied by a fungal species, its pathogenicity and its usefulness as a microbial cell factory to a large degree depends on its secretome. Protein secretion usually requires the presence of a N- terminal signal peptide (SP) and by scanning for this feature using available highly accurate SP-prediction tools, the fraction of potentially secreted proteins can be directly predicted.
However, prediction of a SP does not guarantee that the protein is actually secreted and current in silico prediction methods suffer from gene-model errors introduced during genome annotation.We were able to improve the in silico inventory of A. niger secretory proteins by combining different gene-model predictions from neighbouring Aspergilli and thereby avoiding prediction conflicts associated with inaccurate gene-models. The expected accuracy of signal peptide prediction for proteins that lack homologous sequences in the proteomes of related species is 85%. An experimental validation of the predicted proteome confirmed in silico predictions.

Programme: TransNational Access

SEEK ID: https://hub.ibisba.eu/projects/14

Public web page: Not specified

Organisms: Aspergillus niger

FAIRDOM PALs: No PALs for this Project

Project start date: 1st Jul 2019

Project end date: 31st Dec 2019

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The IBISBA 1.0 project is funded by the EU Horizon 2020 program INFRAIA-02 under grant agreement 730976.