Recombinant nanobody CH10-12 and NbF12-10 production at low induction temperatures and long induction duration
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SEEK ID: https://ibisbahub.eu/studies/159
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Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
Study position: 3
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Created: 18th Apr 2025 at 07:21
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https://orcid.org/0000-0001-8290-1635 
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
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Start date: 6th Feb 2017
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: 1 hidden item
Procedure followed for the data treatment and analysis of 5L bioreactor cultures
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
