5L bioreactor culture of_ E. coli _NbF12-10 at 29°C in nanobody production phase adding 1 mM IPTG to the bioreactor. Caracterization of nanobody production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h
SEEK ID: https://ibisbahub.eu/events/27
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
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21st Feb 2019 at 06:12 (21st Feb 2019 at 07:12 (Europe/Paris))
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Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
Organisms: Escherichia coli
Excel file containing the data anlysis of the BR08 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain _E. coli _NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela