Culture BR08 - induction phase
5L bioreactor culture of_ E. coli _NbF12-10 at 29°C in nanobody production phase adding 1 mM IPTG to the bioreactor. Caracterization of nanobody production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h
SEEK ID: https://ibisbahub.eu/events/27
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
19th Feb 2019 at 16:42 (19th Feb 2019 at 17:42 (Europe/Paris))
21st Feb 2019 at 06:12 (21st Feb 2019 at 07:12 (Europe/Paris))
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Real-time online monitoring of bioprocesses
Industrial biotechnology (IB) has the potential to create new markets while protecting the environment. With this in mind, the EU-funded BIOINDUSTRY 4.0 project will pave the way for IB to become a major manufacturing technology. Supporting the digitalisation of IB, the project will create new services delivered by European research infrastructure. These will address several challenges, focusing on the acceleration of bioprocess development pipelines. ...
Projects: Industrial-scale penicillin simulation, Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH), Production of the monoclonal antibody Trastuzumab in Komagataella phaffii (Pichia pastoris) for breast cancer treatment, Bioindustry4.0 Work Package 7: Tools for high-quality datasets, requisite for data-driven methods and services
Web page: https://www.bioindustry4.eu
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Excel file containing the data anlysis of the BR08 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain _E. coli _NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
