Gas analysis data for BR08 and BR09

Version 1

Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...

In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h

5L bioreactor culture of_ E. coli _NbF12-10 at 29°C in nanobody production phase adding 1 mM IPTG to the bioreactor. Caracterization of nanobody production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h

5L bioreactor culture of E. coli WK6 at 29°C in biomass production phase under induction conditions adding 1 mM IPTG to the bioreactor. Caracterization of biomass production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h

5L bioreactor culture of E. coli NbF12-10 at 37°C in fed-batch phase. Caracterization of biomass production phase in glucose-limited conditions, using an exponential-like glucose feed strategy for a µ=0.38 h-1

5L bioreactor culture of_ E. coli_ WK6 at 37°C in fed-batch phase. Caracterization of biomass production phase in glucose-limited conditions, using an exponential-like glucose feed strategy for a µ=0.38 h-1

5L bioreactor culture of _E. coli _NbF12-10 at 37°C in batch phase. Caracterization of biomass growth

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushing time: 45 s Analysis time: 15 s