Data files

Bioreactor data acquisition - part 1

Bioreactor data acquisition - part 2

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 30°C for 10h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 33°C for 6h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 33°C for 6h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 37°C for 6h

5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 37°C for 6h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h

5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h

5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

Samples taken in the 5L bioreactor BR01 in the culture of E. coli WK6 in batch and fed-batch strategy

Samples taken in the 5L bioreactor BR02 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h

Samples taken in the 5L bioreactor BR03 in the culture of recombinant strain_ E. coli_ CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 30°C for 10 h

Samples taken in the 5L bioreactor BR04 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 33°C for 6 h

Samples taken in the 5L bioreactor BR05 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 37°C for 6 h

Samples taken in the 5L bioreactor BR06 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 38 h

Samples taken in the 5L bioreactor BR07 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 32°C for 35 h

Samples taken in the 5L bioreactor BR08 in the culture of recombinant strain _E. coli _NbF12-10 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 37 h

Samples taken in the 5L bioreactor BR09 in the culture of _E. coli _WK6 in a batch/fed-batch strategy at 37°C. Induction simulation with 1 mM IPTG when biomass reached 25 g cdw/L and at 29°C for 28 h

Excel file containing the data anlysis of the BR01 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR02 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR03 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR04 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR05 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR06 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR07 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR08 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Excel file containing the data anlysis of the BR09 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 4: gas out - BR01 Channel 6: gas in (reference) Sampling time: 60 s Flushing time: 45 s Analysis time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR04 Channel 4: gas out - BR05 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR06 Channel 4: gas out - BR07 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s