ExploreExcel file containing the data anlysis of the BR09 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)
SEEK ID: https://ibisbahub.eu/data_files/168?version=1
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Work-SMAV-BR09-Wild type-F-IBISBA.xlsx
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Created: 13th Jun 2025 at 11:32
Last updated: 13th Jun 2025 at 11:50
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Version 1 (earliest) Created 13th Jun 2025 at 11:32 by Susana Alonso Villela
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https://orcid.org/0000-0001-8290-1635 Bioprocess engineer with extensive expertise in bioreactor process design, fermentation technologies, and protein production. Skilled in scaling up bioprocesses, experimental design, and process optimization.
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
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In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...
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Studies: Characterization of E. coli WK6 strain under aerobic conditions, Characterization of E. coli WK6 strain under induction conditions, Recombinant nanobody CH10-12 and NbF12-10 production at low induction te..., Recombinant nanobody CH10-12 production at different induction temperatu...
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C - BR02, Fed-batch culture of CH10-12 strain, induction at 29°C - BR06, Fed-batch culture of CH10-12 strain, induction at 30°C - BR03, Fed-batch culture of CH10-12 strain, induction at 32°C - BR07, Fed-batch culture of CH10-12 strain, induction at 33°C - BR04, Fed-batch culture of CH10-12 strain, induction at 37°C - BR05, Fed-batch culture of NbF12-10 strain, induction at 29°C - BR08, Fed-batch culture of WK6 strain, induction at 29°C - BR09, Fed-batch culture of WK6 strain, without induction - BR01
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5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
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Assay type: Cultivation Experiment
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5L bioreactor culture of E. coli WK6 at 29°C in biomass production phase under induction conditions adding 1 mM IPTG to the bioreactor. Caracterization of biomass production in glucose-limited conditions, using a constant glucose feed strategy of 4.5 g/h
Start Date: 19th Feb 2019 (19th Feb 2019 (Europe/Paris))
End Date: 20th Feb 2019 (20th Feb 2019 (Europe/Paris))
Event Website: Not specified
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France
City: Toulouse
5L bioreactor culture of_ E. coli_ WK6 at 37°C in fed-batch phase. Caracterization of biomass production phase in glucose-limited conditions, using an exponential-like glucose feed strategy for a µ=0.38 h-1
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5L bioreactor culture of E. coli WK6 at 37°C in batch phase. Caracterization of biomass growth
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5L bioreactor of E. coli WK6 in a batch/fed-batch strategy at 37°C, induction promoted for 28h at 29°C MFCS BioPAT Sartorius
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushing time: 45 s Analysis time: 15 s
