Culture BR09 - batch phase

Excel file containing the data anlysis of the BR09 culture: mass balances, specific growth rate, bioreactor volume, oxygen uptake rate (OUR)

Samples taken in the 5L bioreactor BR09 in the culture of _E. coli _WK6 in a batch/fed-batch strategy at 37°C. Induction simulation with 1 mM IPTG when biomass reached 25 g cdw/L and at 29°C for 28 h

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR08 Channel 4: gas out - BR09 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s