Technology type 'Technology Type'

5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 production at diff...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR02 samples, BioPAT MFCS data for BR02 - part 1, BioPAT MFCS data for BR02 - part 2, Data analysis of the BR02 culture, Gas analysis data for BR02 and BR03

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:16, Last updated: 13th Jun 2025 at 10:35

Fed-batch culture of CH10-12 strain, induction at 30°C - BR03

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 production at diff...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR03 samples, BioPAT MFCS data for BR03 - part 1, BioPAT MFCS data for BR03 - part 2, Data analysis of the BR03 culture, Gas analysis data for BR02 and BR03

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:30, Last updated: 13th Jun 2025 at 10:49

Fed-batch culture of CH10-12 strain, induction at 33°C - BR04

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 production at diff...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR04 samples, BioPAT MFCS data for BR04 - part 1, BioPAT MFCS data for BR04 - part 2, Data analysis of the BR04 culture, Gas analysis data for BR04 and BR05

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:30, Last updated: 13th Jun 2025 at 11:03

Fed-batch culture of CH10-12 strain, induction at 37°C - BR05

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 37°C for 6 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 production at diff...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR05 samples, BioPAT MFCS data for BR05 - part 1, BioPAT MFCS data for BR05 - part 2, Data analysis of the BR05 culture, Gas analysis data for BR04 and BR05

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:31, Last updated: 13th Jun 2025 at 11:07

Fed-batch culture of CH10-12 strain, induction at 29°C - BR06

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 and NbF12-10 produ...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR06 samples, BioPAT MFCS data for BR06 - part 1, BioPAT MFCS data for BR06 - part 2, Data analysis of the BR06 culture, Gas analysis data for BR06 and BR07

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:50, Last updated: 13th Jun 2025 at 11:13

Fed-batch culture of CH10-12 strain, induction at 32°C - BR07

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 and NbF12-10 produ...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR07 samples, BioPAT MFCS data for BR07 - part 1, BioPAT MFCS data for BR07 - part 2, Data analysis of the BR07 culture, Gas analysis data for BR06 and BR07

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:51, Last updated: 13th Jun 2025 at 11:20

Fed-batch culture of NbF12-10 strain, induction at 29°C - BR08

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Recombinant nanobody CH10-12 and NbF12-10 produ...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR08 samples, BioPAT MFCS data for BR08 - part 1, BioPAT MFCS data for BR08 - part 2, Data analysis of the BR08 culture, Gas analysis data for BR08 and BR09

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:51, Last updated: 13th Jun 2025 at 11:27

Fed-batch culture of WK6 strain, induction at 29°C - BR09

Scorpion antivenom nanobody production in Escherichia coli (Recombinant bispecific VHH)

5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h

Submitter: Susana Alonso Villela

Assay type: Cultivation Experiment

Technology type: Fed-batch

Investigation: Effect of the induction temperature in nanobody...

Study: Characterization of E. coli WK6 strain under in...

Organisms: Escherichia coli

SOPs: No SOPs

Data files: BR09 samples, BioPAT MFCS data for BR09 - part 1, BioPAT MFCS data for BR09 - part 2, Data analysis of the BR09 culture, Gas analysis data for BR08 and BR09

Snapshots: No snapshots

Created: 18th Apr 2025 at 08:52, Last updated: 13th Jun 2025 at 11:32