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| Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
| Data File Title: | BR04 samples | ||||||||
| Data File Description: | Samples taken in the 5L bioreactor BR04 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 25 g cdw/L and at 33°C for 6 h | ||||||||
| Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
| Master metadata sheet guide | |||||||||
| Associated Assay | |||||||||
| Existing Assay SEEK ID: | https://ibisbahub.eu/assays/911 | ||||||||
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| New Assay metadata | |||||||||
| Study SEEK ID: | |||||||||
| Assay Title: | |||||||||
| Assay Description: | |||||||||
| Assay type: | Experimental_assay_type | Powered by: | |||||||
| Technology type: | Technology_type | ||||||||
| SOP SEEK ID: | |||||||||
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| Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
| K1 | 22 | 2018-05-29T7:20:0+0000 | 0.028571428571457145 | 0.016923076923051148 | 0.28 | 11.2 | 9.331 | 0.131 | 5.297 | 326 | ||
| K2 | 23 | 2018-05-29T8:20:0+0000 | 0.025000000000005178 | 0.10356321839079397 | 0.44499999999999995 | 11.1 | 9.09 | 0.16 | 5.078 | 326 | ||
| K3 | 22 | 2018-05-29T9:1:0+0000 | 0.1748717948718132 | 0.735 | 9.42 | 8.568 | 0.202 | 4.931 | 326 | |||
| K4 | 22 | 2018-05-29T10:3:0+0000 | 0.482142857142923 | 0.5107407407407478 | 1.51 | 8.99 | 7.67 | 0.28 | 4.774 | 326 | ||
| K5 | 22 | 2018-05-29T10:29:0+0000 | 0.7643333333332928 | 2.2800000000000002 | 7.53 | 7.34 | 0.328 | 4.839 | 326 | |||
| K6 | 12 | 2018-05-29T11:0:0+0000 | 1.1750000000000849 | 1.021500000000053 | 3.02 | 7.6 | 7.658 | 0.381 | 5.4 | 326 | ||
| K7 | 11.5 | 2018-05-29T11:30:0+0000 | 1.5428571428571791 | 1.3699999999999157 | 4.680000000000001 | 6.69 | 5.598 | 0.41 | 4.756 | 326 | ||
| K8 | 11 | 2018-05-29T12:0:0+0000 | 2.749999999999935 | 2.429000000000059 | 5.540000000000001 | 4.76 | 5.012 | 0.528 | 5.683 | 326 | ||
| K9 | 11 | 2018-05-29T12:30:0+0000 | 4.22142857142855 | 3.654000000000046 | 10.600000000000001 | 2.28 | 2.466 | 0.536 | 5.706 | 326 | ||
| K10 | 10 | 2018-05-29T12:59:0+0000 | 5.610714285714247 | 5.055555555555714 | 14.68 | 0.013 | 0.457 | 5.702 | 326 | |||
| K11 | 12 | 2018-05-29T13:53:0+0000 | 8.060714285714278 | 7.131250000000033 | 20.7 | 0.012 | 0.237 | 5.658 | 326 | |||
| K12 | 10 | 2018-05-29T14:50:0+0000 | 12.567857142857164 | 10.30500000000012 | 29.2 | 0.359 | 0.404 | 5.332 | 326 | |||
| K13 | 10 | 2018-05-29T15:20:0+0000 | 15.096428571428504 | 13.580000000000148 | 37.3 | 0.139 | 5.241 | 326 | ||||
| K14 | 10 | 2018-05-29T16:3:0+0000 | 19.028571428571425 | 13.846249999999838 | 47.599999999999994 | 0.012 | 5.111 | 326 | ||||
| K15 | 12 | 2018-05-29T16:41:0+0000 | 25.25357142857137 | 21.97625000000003 | 59.6 | 0.011 | 4.75 | 326 | ||||
| K16 | 55 | 2018-05-29T17:18:0+0000 | 29.528571428571382 | 31.749999999999943 | 72.8 | 0.006 | 4.596 | 0 | 1.2361456351135178 | 326 | ||
| K17 | 50 | 2018-05-29T18:16:0+0000 | 30.714285714285744 | 24.925000000000086 | 81.8 | 0 | 4.63 | 0.07709665792907604 | 0.9580128672129763 | 326 | ||
| K18 | 54 | 2018-05-29T19:22:0+0000 | 31.585714285714314 | 30.55999999999992 | 82.4 | 0.061 | 0.14329953364140965 | 1.3752120190637884 | 326 | |||
| K19 | 52 | 2018-05-29T20:18:0+0000 | 32.96071428571437 | 29.665000000000052 | 83.79999999999998 | 0 | 0.2718821979594908 | 0.9425610467740573 | 326 | |||
| K20 | 53 | 2018-05-29T21:18:0+0000 | 33.56309523809537 | 30.63000000000038 | 85.19999999999999 | 0 | 0.21851746686678242 | 0.7571392015070295 | 326 | |||
| K21 | 48 | 2018-05-29T22:23:0+0000 | 33.39642857142849 | 29.109999999999896 | 88 | 0.006 | 4.403 | 0.25853401508644575 | 0.9734646876518952 | 326 | ||
| K22 | 133 | 2018-05-29T23:16:0+0000 | 34.01785714285709 | 33.500000000000675 | 89.80000000000001 | 0 | 3.881 | 0.4321221359563783 | 1.143434712480004 | 326 | ||
